Informações sobre o curso: This course familiarizes students with the novel concepts being used to revamp regulatory toxicology in response to a breakthrough National Research Council Report “Toxicity Testing in the 21st Century: A Vision and a Strategy.” We present the latest developments in the field of toxicology—the shift from animal testing toward human relevant, high content, high-throughput integrative testing strategies. Active programs from EPA, NIH, and the global scientific community illustrate the dynamics of safety sciences.
Desenvolvido por: Universidade Johns Hopkins
Ministrado por: Lena Smirnova, Research Associate
Center for Alternatives to Animal Testing
Ministrado por: Thomas Hartung, Professor
Environmental Health and Engineering
| Nível | Intermediate |
| Compromisso | 6 weeks of study, 3-6 hours per week |
| Idioma | English |
| Como ser aprovado | Seja aprovado em todas as tarefas para concluir o curso. |
| Classificação do usuário |
Programa
SEMANA 1
Module 1
This model will give you a broad introduction to the Toxicology 21st century (Tox21c) field and familiarize you with the main document, which summarize the main ideas and principles of Tox21c.
10 vídeos, 3 leituras, 1 questionário de prática
- Vídeo: Course Introduction
- Vídeo: Introduction
- Vídeo: Center for Alternatives to Animal Testing
- Vídeo: Toxicology of the Fifteenth to Twentieth Centuries
- Vídeo: Toxicology for the Twenty-First Century
- Reading: useful links
- Reading: supporting reading material
- Vídeo: Historical Perspective
- Vídeo: The First Report: Toxicity Testing for Assessment of Environmental Agents
- Vídeo: The Second Report: Toxicity Testing in the 21st Century: A Vision and A Strategy Vision and Its Components
- Vídeo: The Second Report: Toxicity Testing in the 21st Century: A vision and A Strategy Implementation
- Reading: Supporting reading material
- Practice Quiz: Module 1 Quiz
- Discussion Prompt: Drivers of change
SEMANA 2
Module 2
This module will discuss how ToxCast and Tox21 technologies can be used in practice. US-EPA Endicrone Disruptor Screening Program will be discussed in details and demonstrate how ToxCast platform replaced in vivo tiered approach in EDSP and, thus, acelerate the process of . In second lesson of this module development of in silico model for toxicity testing will be discussed.
10 vídeos, 4 leituras, 1 questionário de prática
- Vídeo: Adverse Outcome Pathways (AOPs)
- Vídeo: Exposure-Based Chemical Prioritization
- Vídeo: ER Bioactivity Model
- Vídeo: US EPA Endocrine Disruptor Screening Program (EDSP)
- Reading: Supporting Reading Material
- Reading: Useful links
- Vídeo: Modeling of Hazard and Organ Manifestation
- Vídeo: Building a Signature for Vascular Disruption
- Vídeo: Testing the Vascular Disruption Signature
- Vídeo: Virtual Tissue Model: Vascular Development
- Vídeo: Functional Vascular Development Assays
- Reading: Supporting Reading Material
- Reading: Useful links
- Practice Quiz: Module 2 Quiz
- Discussion Prompt: Tox21 vs. ToxCast
- Discussion Prompt: AOP
Nota atribuída: Chemical report and endocrine disrpution potency prediction
SEMANA 3
Module 3
This module will familiarize you with Human Toxome Project run by Johns Hopkins University in collaboration with several university, industry and research agencies.
Omics technologies their advantages and disadvantages as well as concepts of Adverse Outcome Pathway (AOP) and Pathway of Toxicity (PoT) will be discussed
11 vídeos, 2 leituras, 1 questionário de prática
- Vídeo: Mechanistic Toxicology and Pathway Mapping at the Basics of 21st Century Toxicology
- Vídeo: The Concept of the Human Toxome Project
- Vídeo: Concept and Tool Devlopment
- Vídeo: Progress
- Vídeo: Conclusions and Prospectives
- Reading: Supporting reading materials
- Vídeo: Introduction - What Is a Pathway of Toxicity, and Why Do We Need Them?
- Vídeo: Omics Technologies and High-Throughput Data
- Vídeo: Interpreting Omics Data
- Vídeo: PoT Examples and Conclusions
- Vídeo: Pathways of Toxicity and Adverse Outcome Pathways (AOPs)
- Reading: Supporting reading material
- Practice Quiz: Module 3 Quiz
- Discussion Prompt: Challenges in implementing omics technologies for high throughput assays
SEMANA 4
Module 4
The first lesson in this module will explain what is the concept of read-across, why it is important for regulatory risk assessment and how it can be implement by industry in response to REACH legislation. Organotypic cultures lesson will teach you about existing in vitro cell-based models starting from simple monolayer cultures of cell lines finishing with complex combined organ-on-a-chip and human-on-a-chip approaches.
10 vídeos, 3 leituras, 1 questionário de prática
- Vídeo: Introduction to Read-Across
- Vídeo: Good Read-Across Practice
- Vídeo: Supporting Read-Across with Biological Data
- Vídeo: Creating a Database of Toxicity
- Vídeo: Creating an Automated Tool for Read-Across
- Reading: Supporting reading material
- Vídeo: Paradigm Shift in Toxicology
- Vídeo: 2D In Vitro Cell Culture
- Vídeo: 3D In Vitro Cell Cultures
- Vídeo: Organ-on-a-Chip
- Reading: Supporting reading material
- Reading: Useful links
- Practice Quiz: Module 4 Quiz
- Discussion Prompt: Read-across
Nota atribuída: Mid-term exam
Module 5
This module will teach you the role of miRNA and epigenetics in toxicology and environmental health.
6 vídeos, 1 leitura, 1 questionário de prática
- Vídeo: MicroRNA and Epigenetics
- Vídeo: Epigenetics and Environment
- Vídeo: MicroRNA: Biogenesis and Functions
- Vídeo: Role of MicroRNA in Development
- Vídeo: MicroRNA in Toxicology
- Reading: Supporting reading material
- Practice Quiz: Module 5 Quiz
- Discussion Prompt: MicroRNAs potential to be biomarkers for diseases and toxicity
SEMANA 5
Module 6
In this module you will learn about important component of in vitro toxicology such as In Vitro to In Vivo Extrapolation and physiologically based biokinetic modeling. You will learn why it is important to consider biokinetics in vitro and see some example of biokinetics modeling in vitro. Also this lesson will give you a biometry prospective of in vitro toxicology. You will learn which methods can be used to analyze the big data.
8 vídeos, 3 leituras, 1 questionário de prática
- Vídeo: Physiologically Based Biokinetic Modeling
- Vídeo: Application of QIVIVE to in Vitro Testing
- Vídeo: Challenges for Conducting QIVIVE
- Vídeo: Quantitative in Vitro to in Vivo Extrapolation
- Reading: Supporting reading material
- Vídeo: Definition of Biometry/Biostatistics and Revisiting Some Statistical Basics
- Vídeo: Types of Tox21 Data and Data Mining of External Data Sources
- Vídeo: Bioinformatics Tools for the Interpretation of Tox21 Data
- Reading: Supporting reading material
- Reading: Useful links
- Practice Quiz: Module 6 Quiz
Nota atribuída: Peer Assessment 2: Summarizing Your Knowledge on Tools and Applications in Toxicology in the 21st Century
SEMANA 6
Module 7
In this lesson you will learn about integrated testing strategies and how they differ from individual tests or test batteries. Also a wrap-up lesson "beyond chemical" will summaries your knowledge from this course and give a out-look for further directions in the field of alternatives to animal testing.
11 vídeos, 2 leituras, 1 questionário de prática
- Vídeo: Why Integrated Testing Strategies?
- Vídeo: What is an Integrated Testing Strategy?
- Vídeo: How to Compose Integrated Testing Strategies
- Vídeo: Strategies for Integrated Testing Strategies
- Vídeo: Quality Assurance of integrated Testing Strategies
- Reading: Supporting reading material
- Vídeo: Legal Background
- Vídeo: Economic Background
- Vídeo: Tox-21c for Drug Safety
- Vídeo: Green Toxicology as Non-regulatory Tox-21c Approaches
- Vídeo: Conclusions and Prospects
- Reading: Supporting reading material
- Practice Quiz: Module 7 Quiz
Nota atribuída: Final Exam
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SK
Very nicely designed course. The instructors offer an excellent and easy to understand overview of a range of promising Tox21 tools and approaches using good examples and illustrations.