Informações sobre o curso
4.6
164 ratings
40 reviews
This course will cover the topic of Whole genome sequencing (WGS) of bacterial genomes which is becoming more and more relevant for the medical sector. WGS technology and applications are high on international political agenda, as the classical methods are being replaced by WGS technology and therefore bioinformatic tools are extremely important for allowing the people working in this sector to be able to analyze the data and obtain results that can be interpreted and used for different purposes. The course will give the learners a basis to understand and be acquainted with WGS applications in surveillance of bacteria including species identification, typing and characterization of antimicrobial resistance and virulence traits as well as plasmid characterization. It will also give the opportunity to learners to learn about online tools and what they can be used for through demonstrations on how to use some of these tools and exercises to be solved by learners with use of freely available WGS analysis tools . By the end of this course you should be able to: 1. Describe the general Principles in typing of Bacteria 2. Give examples of the applications of Whole Genome Sequencing to Surveillance of bacterial pathogens and antimicrobial resistance 3. Apply genomic tools for sub-typing and surveillance 4. Define the concept of Next-Generation Sequencing and describe the sequencing data from NGS 5. Describe how to do de novo assembly from raw reads to contigs 6. Enumerate the methods behind the tools for species identification, MLST typing and resistance gene detection 7. Apply the tools for species identification, MLST typing and resistance gene detection in real cases of other bacterial and pathogen genomes. 8. Describe the methods behind the tools for Salmonella and E.coli typing, plasmid replicon detection and plasmid typing 9. Utilize the tools for Salmonella and E.coli typing, plasmid replicon detection and plasmid typing in real cases of other bacterial and pathogen genomes. 10. Explain the concept and be able to use the integrated bacterial analysis pipeline for batch analysis and typing of genomic data 11. Demonstrate how to construct phylogenetic tree based on SNPs 12. Apply the phylogenetic tool to construct phylogenetic trees and explain the relatedness of bacterial or pathogen strains 13. Describe how to create your own sequence database 14. Utilize the MyDbFinder tool to detect genetic markers of interest from whole genome sequencing...
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Beginner Level

Nível iniciante

Clock

Sugerido: This course will run over 5 weeks, 2-3 hours a week

Aprox. 10 horas restantes
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English

Legendas: English

Habilidades que você terá

GenomeMicrobiologyAntimicrobialNucleotide
Globe

cursos 100% online

Comece imediatamente e aprenda em seu próprio cronograma.
Calendar

Prazos flexíveis

Redefinir os prazos de acordo com sua programação.
Beginner Level

Nível iniciante

Clock

Sugerido: This course will run over 5 weeks, 2-3 hours a week

Aprox. 10 horas restantes
Comment Dots

English

Legendas: English

Programa - O que você aprenderá com este curso

1

Seção
Clock
1 hora para concluir

Module 1

Welcome and introduction to typing of bacteria and use of Whole genome sequencing applied to surveillance of bacterial pathogens and antimicrobial resistance...
Reading
3 vídeos (Total de 32 min), 1 teste
Video3 videos
General Principles of typing of bacteria12min
Surveillance of Antimicrobial Resistance using whole genome sequencing12min
Quiz1 exercício prático
Module 120min

2

Seção
Clock
1 hora para concluir

Module 2

Introduction to Next Generation sequencing...
Reading
3 vídeos (Total de 37 min), 1 teste
Video3 videos
Introduction to Next-Generation sequencing (NGS)19min
De novo assembly- from raw reads to contigs: assembler tool description and application5min
Quiz1 exercício prático
Module 230min

3

Seção
Clock
1 hora para concluir

Module 3

Whole genome sequencing tools- demonstration of analysis tools for species identification, MLST typing and finding resistance genes...
Reading
3 vídeos (Total de 30 min), 1 teste
Video3 videos
MLST Typing: MLST tool description and applications8min
Resistance gene detection: Resfinder tool description and applications12min
Quiz1 exercício prático
Module 320min

4

Seção
Clock
1 hora para concluir

Module 4

Whole genome sequencing tools- demonstration of analysis tools for Serotyping of Salmonella and Escherichia coli strains , and finding plasmid replicons...
Reading
3 vídeos (Total de 24 min), 1 teste
Video3 videos
E. coli serotype identification: serotype finder tool description and applications6min
Plasmid replicon identification and plasmid typing12min
Quiz1 exercício prático
Module 420min
4.6
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83%

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Melhores avaliações

por PTSep 17th 2017

Thank you great team from DTU, I get to understand NGS via your detailed explanation and useful demonstration as well as example exercise. Cheers

por SKMay 18th 2018

The contents of the course were really very good and with loads of knowledge. Thank you very much for improving my knowledge in this area.

Instrutores

Lina Cavaco

Ext Researcher
Copenhagen University- ICMM /Statens Serum Institut - Department for Bacteria, Parasites and Fungi

Pimlapas Leekitcharoenphon

PostDoc
Research Group for Genomic Epidemiology, DTU Food

Sobre Technical University of Denmark (DTU)

DTU focuses on research in technical and natural sciences that contributes to the development of society. As an industrially orientated university, our goal is to supply high-level international research based on combining theory with constructing models and empirical methods....

Perguntas Frequentes – FAQ

  • Once you enroll for a Certificate, you’ll have access to all videos, quizzes, and programming assignments (if applicable). Peer review assignments can only be submitted and reviewed once your session has begun. If you choose to explore the course without purchasing, you may not be able to access certain assignments.

  • When you purchase a Certificate you get access to all course materials, including graded assignments. Upon completing the course, your electronic Certificate will be added to your Accomplishments page - from there, you can print your Certificate or add it to your LinkedIn profile. If you only want to read and view the course content, you can audit the course for free.

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