Loading...

筛选特定表型或者信号通路相关基因实例(2)

正向遗传学策略,以EMS诱变果蝇,筛选两类表型:1. 睡眠异常表型;2.根据翅的表型筛选与Notch、Hedgehog以及Wingless信号通路相关的基因。然后以平衡染色体为路标工具,确定突变的基因是什么。反向遗传学策略,针对携带P因子插入的基因品系,通过遗传杂交,获得该基因的突变体。进一步对突变体的功能进行研究:通过rescue实验确认基因突变与表型的特异性的对应关系;确定内源基因表达在哪里(原位杂交, 抗体染色等);基因间的相互作用,上下游关系的确定;细胞(神经元)间的相互作用等。

Vídeos recomendados

Sobre o Coursera

Courses, Specializations, and Online Degrees taught by top instructors from the world's best universities and educational institutions.

Community
Join a community of 40 million learners from around the world
Certificate
Earn a skill-based course certificate to apply your knowledge
Career
Gain confidence in your skills and further your career