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Voltar para Basics of Extracellular Vesicles

Comentários e feedback de alunos de Basics of Extracellular Vesicles da instituição Universidade da Califórnia, Irvine

4.8
stars
168 classificações
•
42 avaliações

Sobre o curso

This course aims to provide the basic knowledge about extracellular vesicles (EV) a generic term including exosomes, microvesicles, microparticles, ectosomes, oncosomes, prostasomes, and many others. It covers areas such as EV history, nomenclature, biogenesis, EV cargo as well as the release and uptake mechanisms, collection and processing prior to isolation, different isolation methods, characterization and quantification techniques. This course is divided into five modules. Module 1 is an introduction to the field and will cover the nomenclature and the history of EVs. Module 2 will focus on the biogenesis, release and uptake mechanisms of EVs as well as the different EV cargos (RNA, protein, lipids). In Module 3, we will focus on the collection and processing of cell culture media and body fluids such as blood, breast milk, cerebrospinal fluid and urine prior to isolation of EVs. Module 4 and 5 will present different isolation methods and characterization/quantification techniques, respectively. Here differential ultracentrifugation, size exclusion chromatography, density gradient, kit based precipitation, electron microscopy (EM), cryo-TEM, flow cytometry, atomic-force microscopy and nanoparticle tracking analysis will be presented. The recommended prerequisites are college-freshman-level biology and biochemistry. After a completed course you should be able to: + Discuss the nomenclature and subgroups of extracellular vesicles. + Describe the RNA, protein and lipid content of extracellular vesicles. + Describe the basic concepts about the most common isolation and characterization techniques and how these techniques are used in the EV field. + State the benefits and limitations of the most common isolation and characterization techniques for extracellular vesicles. + Explain the considerations that are important during the collection and isolation of EVs from different body fluid. + Describe the release and uptake mechanisms of extracellular vesicles All lectures are given in English. Each of the five modules will be followed by an exam. All exams will be in the format of multiple choice questions. The course is organized in collaboration between the International Society for Extracellular Vesicles (ISEV), University of California Irvine (USA), University of Gothenburg (Sweden) and Pohang University of Science and Technology (South Korea)....

Melhores avaliações

MH

Jan 11, 2019

Awosome course. Lot of information given with paper based data and suggestion. I really love it. I am also working with Exosome. I use cell culture media from PC12 and fibroblast cell line.

MV

Jan 31, 2018

The only problem I faced, has to do with the subtitles. They have lots of mismatches and mistakes. Nevertheless, the MOOC is undoubtedly great and pretty educational.

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26 — 39 de {totalReviews} Avaliações para o Basics of Extracellular Vesicles

por Stephanie B

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Oct 05, 2019

Good basic overview for someone starting in their studies

por Wenhao L

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Apr 26, 2017

Very wonderful courses! Better than reading 100 papers!

por Mohammad R

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Feb 05, 2017

This course gives an excellent overview of the field!

por pbmansfield

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Mar 06, 2017

Well put together and very informative. Great job!

por Abner M N

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Nov 22, 2019

Amazing course!

por Adel D

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Nov 03, 2016

very good!

por Kaio M R

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Oct 10, 2017

ÓTIMO

por Mainak B

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Nov 02, 2019

Excellent content for learning about basic of extracellular vesicles beginners. One thing I would like to point out is it would have been great if the course materials are made available as a pdf version.

por Enrique V O

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May 11, 2017

It is good much info about vesicles that you can't get from the books and techniques to study them.

por Shalmali P

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Mar 03, 2018

Very nice nd inormative presentations. But few words in many presentations are not audible.

por David d V L

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Mar 29, 2018

Excellents classes I have learned a lot of procotols about EVs

por Xiaokun L

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Jul 20, 2017

nice

por lcy9086

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Apr 01, 2018

I am sorry to say that many parts of the lecture provided by the Korean professor is inaudible......SO you can only learn from his slides :(

por Shukhman B

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Nov 12, 2016

The most important benefit of this course is the fact that it exists. At present the course is mediocre to bad: its educational value is low: transcripts are disgusting, they are not proofread, omitted pats of sentences, inconsistent English, they almost completely obscure, disrupt the sense of the presentation. The course offers no slides. I see obvious negligence in preparation of many lectures (especially bad week three).

The target audience for the course is professional biologist that are comparable with arithmetic and elementary algebra and can easily use an internet browser.

The course contains numerous mistakes: in particular, the elementary formula Dr. Cecilia Lasser presented in her week 4 lecture on Differential Ultracentrifugation contains an error.

The lectures presented by non-native English speakers are very difficult to understand because of poor & inconsistent English.

Please, make the lecture slide available for your students.

Please, arrange a forum giving a possibility to ask a questions and get understanding of the material that remains unclear.

The lectures on electron microscopy were very good, - thank you, Johanna!

The lectures on AFM was the only one that was just excellent, - Thank you, Dr. Shivani Sharma.

Please, review the sequence of lectures: I think the week 5 material may be moved to week 3.