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Voltar para Basics of Extracellular Vesicles

Comentários e feedback de alunos de Basics of Extracellular Vesicles da instituição Universidade da Califórnia, Irvine

4.8
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316 classificações
•
88 avaliações

Sobre o curso

This course aims to provide the basic knowledge about extracellular vesicles (EV) a generic term including exosomes, microvesicles, microparticles, ectosomes, oncosomes, prostasomes, and many others. It covers areas such as EV history, nomenclature, biogenesis, EV cargo as well as the release and uptake mechanisms, collection and processing prior to isolation, different isolation methods, characterization and quantification techniques. This course is divided into five modules. Module 1 is an introduction to the field and will cover the nomenclature and the history of EVs. Module 2 will focus on the biogenesis, release and uptake mechanisms of EVs as well as the different EV cargos (RNA, protein, lipids). In Module 3, we will focus on the collection and processing of cell culture media and body fluids such as blood, breast milk, cerebrospinal fluid and urine prior to isolation of EVs. Module 4 and 5 will present different isolation methods and characterization/quantification techniques, respectively. Here differential ultracentrifugation, size exclusion chromatography, density gradient, kit based precipitation, electron microscopy (EM), cryo-TEM, flow cytometry, atomic-force microscopy and nanoparticle tracking analysis will be presented. The recommended prerequisites are college-freshman-level biology and biochemistry. After a completed course you should be able to: + Discuss the nomenclature and subgroups of extracellular vesicles. + Describe the RNA, protein and lipid content of extracellular vesicles. + Describe the basic concepts about the most common isolation and characterization techniques and how these techniques are used in the EV field. + State the benefits and limitations of the most common isolation and characterization techniques for extracellular vesicles. + Explain the considerations that are important during the collection and isolation of EVs from different body fluid. + Describe the release and uptake mechanisms of extracellular vesicles All lectures are given in English. Each of the five modules will be followed by an exam. All exams will be in the format of multiple choice questions. The course is organized in collaboration between the International Society for Extracellular Vesicles (ISEV), University of California Irvine (USA), University of Gothenburg (Sweden) and Pohang University of Science and Technology (South Korea)....

Melhores avaliações

MH

10 de jan de 2019

Awosome course. Lot of information given with paper based data and suggestion. I really love it. I am also working with Exosome. I use cell culture media from PC12 and fibroblast cell line.

DD

16 de mai de 2020

I found Ia very professional way to introduce everyone to the amazing world of EV. Professor show elegantly their knowledge. The course fulfils my expectations. Congratulations! Nice works

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76 — 85 de 85 Avaliações para o Basics of Extracellular Vesicles

por Mainak B

•

2 de nov de 2019

Excellent content for learning about basic of extracellular vesicles beginners. One thing I would like to point out is it would have been great if the course materials are made available as a pdf version.

por Enrique V O

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11 de mai de 2017

It is good much info about vesicles that you can't get from the books and techniques to study them.

por Shalmali P

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3 de mar de 2018

Very nice nd inormative presentations. But few words in many presentations are not audible.

por Marine H

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11 de ago de 2020

The last two modules were extremely helpful and a very good opener to this topic.

por David d V L

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29 de mar de 2018

Excellents classes I have learned a lot of procotols about EVs

por paula s m c

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4 de abr de 2022

Es un curso muy completo y bien orientado.

por Xiaokun L

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20 de jul de 2017

nice

por Alexander Z

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7 de out de 2020

Overall, pretty good background information. But some of these lectures need to be a bit more audible. I only had one issue with a quiz in which it felt like the question asked either wasn't covered or emphasized as important to the overall subject.

por lcy9086

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1 de abr de 2018

I am sorry to say that many parts of the lecture provided by the Korean professor is inaudible......SO you can only learn from his slides :(

por Yusuf M A

•

21 de abr de 2021

Cannot understand the instructors.

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