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Voltar para Basics of Extracellular Vesicles

Comentários e feedback de alunos de Basics of Extracellular Vesicles da instituição Universidade da Califórnia, Irvine

4.8
estrelas
316 classificações
88 avaliações

Sobre o curso

This course aims to provide the basic knowledge about extracellular vesicles (EV) a generic term including exosomes, microvesicles, microparticles, ectosomes, oncosomes, prostasomes, and many others. It covers areas such as EV history, nomenclature, biogenesis, EV cargo as well as the release and uptake mechanisms, collection and processing prior to isolation, different isolation methods, characterization and quantification techniques. This course is divided into five modules. Module 1 is an introduction to the field and will cover the nomenclature and the history of EVs. Module 2 will focus on the biogenesis, release and uptake mechanisms of EVs as well as the different EV cargos (RNA, protein, lipids). In Module 3, we will focus on the collection and processing of cell culture media and body fluids such as blood, breast milk, cerebrospinal fluid and urine prior to isolation of EVs. Module 4 and 5 will present different isolation methods and characterization/quantification techniques, respectively. Here differential ultracentrifugation, size exclusion chromatography, density gradient, kit based precipitation, electron microscopy (EM), cryo-TEM, flow cytometry, atomic-force microscopy and nanoparticle tracking analysis will be presented. The recommended prerequisites are college-freshman-level biology and biochemistry. After a completed course you should be able to: + Discuss the nomenclature and subgroups of extracellular vesicles. + Describe the RNA, protein and lipid content of extracellular vesicles. + Describe the basic concepts about the most common isolation and characterization techniques and how these techniques are used in the EV field. + State the benefits and limitations of the most common isolation and characterization techniques for extracellular vesicles. + Explain the considerations that are important during the collection and isolation of EVs from different body fluid. + Describe the release and uptake mechanisms of extracellular vesicles All lectures are given in English. Each of the five modules will be followed by an exam. All exams will be in the format of multiple choice questions. The course is organized in collaboration between the International Society for Extracellular Vesicles (ISEV), University of California Irvine (USA), University of Gothenburg (Sweden) and Pohang University of Science and Technology (South Korea)....

Melhores avaliações

MH

10 de jan de 2019

Awosome course. Lot of information given with paper based data and suggestion. I really love it. I am also working with Exosome. I use cell culture media from PC12 and fibroblast cell line.

DD

16 de mai de 2020

I found Ia very professional way to introduce everyone to the amazing world of EV. Professor show elegantly their knowledge. The course fulfils my expectations. Congratulations! Nice works

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51 — 75 de 85 Avaliações para o Basics of Extracellular Vesicles

por Daniel .

3 de abr de 2022

It´s a great course to people that starting to study of vesicles.

por Asmaa A M A

14 de mai de 2020

Excellent course, totally enjoyed it and benefited alot from it

por Laura M M E

4 de mai de 2020

Excelente curso, expositores con amplio conocimiento del tema

por Stephanie B

5 de out de 2019

Good basic overview for someone starting in their studies

por Wenhao L

26 de abr de 2017

Very wonderful courses! Better than reading 100 papers!

por Mohammad R

5 de fev de 2017

This course gives an excellent overview of the field!

por Nahla I S H

22 de abr de 2020

A very good course, really it was very informative.

por pbmansfield

6 de mar de 2017

Well put together and very informative. Great job!

por Dr.� S R S A P -

26 de jun de 2020

very helpful and nice videos and the contents too

por Ramiro R d l C

4 de nov de 2020

Great! Very informative and I learned a lot.

por Emilio E S

26 de jul de 2021

really good course. I learn a lot about EVs

por Regina C

29 de dez de 2020

Very interesting and simple to understand

por Olivia C

6 de ago de 2020

very useful course, I really enjoy it!

por Yingchao M

9 de abr de 2020

Very good for beginners.

por David H

1 de set de 2020

excellent course

por Ábner M N

21 de nov de 2019

Amazing course!

por Dr T S R

17 de set de 2020

Super Course

por Adel D

3 de nov de 2016

very good!

por CAROLINA T P

30 de jul de 2021

Excellent

por ganesha p

16 de set de 2020

Excellent

por Lee-Ann M C

9 de jul de 2021

Usefull

por Kaio M R

9 de out de 2017

ÓTIMO

por Emayamsavitha

27 de abr de 2020

Gud

por PRIYASHREE N

3 de jul de 2020

The way the course was introduced and was taught is really amazing. There were few issues in understanding the audio and there was problem with subtitles as well but still it was a good and amazing experience to meet and listen to researchers from various parts of world. Though it is a blooming field, there was a lot of information in this course. And I gained lot of information from this course. Thanks for every single researcher and professor who are involved in this course. It was a great pleasure to meet and hear your lectures.

por Arjun J

7 de set de 2021

Definitely got to learn quite a bit of possibly useful stuff (I'm very new to this) during the course. The quality of lectures varied on a spectrum from average to great, depending on the instructor for each topic. While some of them literally just read off the slides they prepared, others delivered the info with evident passion, which kept you attentive and interested. It's a mixed bag, but don't let that stop you - overall, the course experience was not bad.