Informações sobre o curso
4.7
118 classificações
29 avaliações
100% online

100% online

Comece imediatamente e aprenda em seu próprio cronograma.
Prazos flexíveis

Prazos flexíveis

Redefinir os prazos de acordo com sua programação.
Nível intermediário

Nível intermediário

Horas para completar

Aprox. 13 horas para completar

Sugerido: 5 weeks, 2-3 hours/week...
Idiomas disponíveis

Inglês

Legendas: Inglês

Habilidades que você terá

BiologySample PreparationSample CollectionCell Biology
100% online

100% online

Comece imediatamente e aprenda em seu próprio cronograma.
Prazos flexíveis

Prazos flexíveis

Redefinir os prazos de acordo com sua programação.
Nível intermediário

Nível intermediário

Horas para completar

Aprox. 13 horas para completar

Sugerido: 5 weeks, 2-3 hours/week...
Idiomas disponíveis

Inglês

Legendas: Inglês

Programa - O que você aprenderá com este curso

Semana
1
Horas para completar
2 horas para concluir

Introduction to the Course and the Field of Extracellular Vesicles

In this module, we will introduce the course and the field of extracellular vesicles. We will discuss the nomenclature and the history of extracellular vesicles as well as the organisms and places that have been shown to release/contain extracellular vesicles. ...
Reading
4 videos (Total 69 min), 1 leitura, 1 teste
Video4 videos
Introduction to the Course11min
The Origin of EVs throughout the Phylogenetic Tree11min
History of Exosomes and Extracellular Vesicles32min
Reading1 leituras
Syllabus10min
Quiz1 exercícios práticos
Introduction to Extracellular Vesicles18min
Semana
2
Horas para completar
3 horas para concluir

Biogenesis, Cargo and Uptake of Extracellular Vesicles

In this module, we will focus on the biogenesis and release of extracellular vesicles as well as the uptake mechanisms of extracellular vesicles when they are encountered by a recipient cell. This module also covers the different RNA, protein and lipid types present in extracellular vesicles as well as a brief overview what the functions of these molecules are. Furthermore, the techniques that are commonly used to detect these molecules as well as databases that can be useful to use when analyzing the cargo of extracellular vesicles will be highlighted....
Reading
6 videos (Total 142 min), 1 teste
Video6 videos
Mechanisms of Extracellular Vesicle Uptake - Part 122min
Mechanisms of Extracellular Vesicle Uptake - Part 223min
The Protein Content of Extracellular Vesicles23min
The RNA Content of Extracellular Vesicles35min
The Lipid Content of Extracellular Vesicles18min
Quiz1 exercícios práticos
Biogenesis, Cargo and Uptake of Extracellular Vesicles30min
Semana
3
Horas para completar
2 horas para concluir

Collection and Processing of Cell Culture Media and Body Fluids Prior to Isolation of Extracellular Vesicles

In this module, we will focus on the collection and processing of cell culture media and body fluids prior to isolation of extracellular vesicles. We will discuss some of the considerations and guidelines that are important during the collection of staring material and isolation of extracellular vesicles. This module will help you with the different choices, such as anticoagulants, collection time points and protein inhibitors, which are important for the outcome when working with a particular body fluid compared to conditional media or other body fluids. This module will also illustrate some example of studies on extracellular vesicles from body fluid such as blood, urine, breast milk and cerebrospinal fluid and why it is of interest to analyse extracellular vesicles from these body fluids. ...
Reading
5 videos (Total 79 min), 1 teste
Video5 videos
Blood Plasma and Serum15min
Breast Milk8min
Urine25min
Cerebrospinal Fluid15min
Quiz1 exercícios práticos
Collection and Processing of Cell Culture Media and Body Fluids Prior to Isolation of Extracellular Vesicles36min
Semana
4
Horas para completar
2 horas para concluir

Methods for Isolating Extracellular Vesicles

This module highlights some of the different methods used to isolate extracellular vesicles. Here the basic concepts for methods such as differential ultracentrifugation, density gradient, size exclusion and kit-based precipitation will be covered and some guidelines will be presented. Furthermore, this module will cover how the techniques are used in the field of extracellular vesicles as well as their limitations and benefits....
Reading
6 videos (Total 77 min), 1 teste
Video6 videos
Differential Ultracentrifugation - Part 216min
Density Gradient12min
Size Exclusion Chromatography14min
Kit-based Precipitation12min
Summary of Isolation Methods for Extracellular Vesicles6min
Quiz1 exercícios práticos
Methods for Isolating Extracellular Vesicles22min

Instrutores

Avatar

Cecilia Lasser

Researcher
Krefting Research Centre, University of Gothenburg

Sobre University of California, Irvine

Since 1965, the University of California, Irvine has combined the strengths of a major research university with the bounty of an incomparable Southern California location. UCI’s unyielding commitment to rigorous academics, cutting-edge research, and leadership and character development makes the campus a driving force for innovation and discovery that serves our local, national and global communities in many ways....

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