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Voltar para Métodos experimentais em biologia sistêmica

Comentários e feedback de alunos de Métodos experimentais em biologia sistêmica da instituição Escola de Medicina Icahn do Hospital Monte Sinai

281 classificações
46 avaliações

Sobre o curso

Learn about the technologies underlying experimentation used in systems biology, with particular focus on RNA sequencing, mass spec-based proteomics, flow/mass cytometry and live-cell imaging. A key driver of the systems biology field is the technology allowing us to delve deeper and wider into how cells respond to experimental perturbations. This in turns allows us to build more detailed quantitative models of cellular function, which can give important insight into applications ranging from biotechnology to human disease. This course gives a broad overview of a variety of current experimental techniques used in modern systems biology, with focus on obtaining the quantitative data needed for computational modeling purposes in downstream analyses. We dive deeply into four technologies in particular, mRNA sequencing, mass spectrometry-based proteomics, flow/mass cytometry, and live-cell imaging. These techniques are often used in systems biology and range from genome-wide coverage to single molecule coverage, millions of cells to single cells, and single time points to frequently sampled time courses. We present not only the theoretical background upon which these technologies work, but also enter real wet lab environments to provide instruction on how these techniques are performed in practice, and how resultant data are analyzed for quality and content....

Melhores avaliações


29 de nov de 2020

Amazing course, only the final exam was rather an excercise in finding out out where the answers differ than trying to really understand what should be the ringht answer. I learnt a lot, thank you!


20 de jun de 2021

The lab videos are great, thanks for teaching us. Not all university can afford those machines. If you have latest methods or anything please introduce more courses.

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1 — 25 de 46 Avaliações para o Métodos experimentais em biologia sistêmica

por Paul G

31 de mar de 2020

Whilst a significant improvement on the dreadful first course in the specialisation, I found this course frustrating and poor in several key areas. Firstly, I am a Biomedical Informatician with several years hands-on experience and am looking to build up my skills in Systems Biology, and I hoped this specialisation would help with that. Based on the first two courses, it doesn't - at all.

Firstly, whilst the lecture videos are much better than in the first course, it really adds little to investigate lab procedures in such detail. An overview of how they work and a focus *on the data produced* and to how that are relevant to models based upon differential equations would have been excellent: this only happens in the final week! The instructor has never posted once in the discussion forums since the course began and there are issues reported with the quizzes which appear to not have been rectified - so the quality control is poor and I found for me personally, much of the material was irrelevant to my aims and objectives in taking the course.

Coursera really have missed an opportunity with this specialisation: the MIT Synthetic Biology course offered by edX is excellent, even though it requires a very high degree of background knowledge it doesn't shy away from the materials (it required too much Molecular Biology for me though it's an excellent course). "Experimental Methods in Systems Biology" *could* have been excellent - IF the material of the final week of lectures had been the majority of the coure. Very disappointing and, honestly, a waste of money in my honest opinion.

por Esteban C

8 de mai de 2020

Un excelente curso que da un panorama muy útil de las herramientas comúnmente empleadas para experimentos enfocados a la biología de sistemas

por Felix E R P

3 de jun de 2016

Excellent course with a wealth of information about system biology technologies

por Eduardo R M

4 de dez de 2015

Great course,

Recommended if you are interest in new technologies

por Sheetal J

22 de set de 2019

Loved this course. Prof. Birtwistle is really into teaching and liked the way he has organized this course. Thanks!

por Guolong M

3 de jan de 2021

3stars for the lab video, -1 for quizzes, -1 for the long lectures

This course has the best lab videos to offer. I have real experience for most of these techniques so I should be qualified to say that the course is extremely helpful to those who will touch on these stuffs. The concepts and the principles of techniques are covered comprehensively but could have been more concise; the lectures are generally too long. The major downside, as others have pointed out, is that the quizzes are not well-organized. There are too many confusing multiple choice problems; the tester clearly did not know the choice should be independent. I've passed this course with 100% grades (with a lot of attempts and trying) Some answers are clearly incorrect if you lookup the literature; the tester should consider some techniques would be improved overtime and therefore some limitation will be lifted, but this course will stay on coursera forever). Furthermore, some problems are not covered or not emphasized in the lectures.

por Himanshu G

21 de fev de 2019

A good course for someone interested to work in the field of systems biology, and thanks to Icahn School of Medicine and Coursera for the same.

But the course could have been a bit more comprehensive with respect to cellular experiments and include coverage of experiments done outside the Mount Sinai lab as well, especially 'non-omic' experiments such as cell growth and viability assays or KD , KO or Over-Expression studies that can be really useful for building network models.

por Joyce C M A

12 de mai de 2019

This course is definitely not for beginners and students must have previous lab experience (in my opinion). It is a great course to get your feet wet in new techniques that you have not performed yet.

por Harshit K S

4 de mai de 2020

A really nice course which I believe served its purpose. Maybe a pdf version of experimental protocols followed in the lab videos can be added in the reading section of the lab-based video or in optional reading. This will help students who are interested in knowing the specific protocols for the technique.

por Adam H

29 de nov de 2020

Amazing course, only the final exam was rather an excercise in finding out out where the answers differ than trying to really understand what should be the ringht answer. I learnt a lot, thank you!

por julio c r m

7 de ago de 2016

This a great course it helps a lot to get to know state of the art techniques and how to use them properly although the assigment questions of selecting the statements are quite confusing.

por Yuridia M

8 de jun de 2020

Excelente curso, en lo personal me dio más perspectivas para el trabajo con sistemas biológicos. Me gustaría que se profundizara más sobre el tema de redes metabólicas y análisis de datos.

por Mimi D

21 de jun de 2021

T​he lab videos are great, thanks for teaching us. Not all university can afford those machines. If you have latest methods or anything please introduce more courses.

por Luna Z

9 de jan de 2021

Very difficult, would help if instructor provides answer key or monitors the chat a bit more

por Mark E S

30 de set de 2020

Awesome delivery and exam questions are very practical....I enjoyed the 8weeks here

por Vitória S B

10 de jul de 2020

I relly enjoy and learn so much with that course! I recommend :)

por Cristina B

24 de mai de 2020

excellent overview of experimental methods in systems biology

por Jiho C

9 de mar de 2017

Good lecture to know experimental method in lab


5 de jun de 2022

It was quite knowledgeble

por Boudalia M E A

15 de jan de 2016



por Yingchao M

20 de abr de 2020

A very good course.

por Mac D G

24 de jan de 2018

it was so useful

por Dominika B

28 de set de 2016

Very useful. :)

por farzaneh d d

6 de set de 2016

its very good.

por Nurşen Ç

10 de jun de 2019

ain't better!