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Voltar para Introdução à Crio-Microscopia Eletrônica (Crio-ME)

Comentários e feedback de alunos de Introdução à Crio-Microscopia Eletrônica (Crio-ME) da instituição Caltech – Instituto de Tecnologia da CalifórniaCaltech - Instituto de Tecnologia da Califórnia

266 classificações
51 avaliações

Sobre o curso

This class covers the fundamental principles underlying cryo-electron microscopy (cryo-EM) starting with the basic anatomy of electron microscopes, an introduction to Fourier transforms, and the principles of image formation. Building upon that foundation, the class then covers the sample preparation issues, data collection strategies, and basic image processing workflows for all 3 basic modalities of modern cryo-EM: tomography, single particle analysis, and 2-D crystallography. Philosophy: The course emphasizes concepts rather than mathematical details, taught through numerous drawings and example images. It is meant for anyone interested in the burgeoning fields of cryo-EM and 3-D EM, including cell biologists or molecular biologists without extensive training in mathematics or imaging physics and practicing electron microscopists who want to broaden their understanding of the field. The class is perfect as a primer for anyone who is about to be trained as a cryo-electron microscopist, or for anyone who needs an introduction to the field to be able to understand the literature or the talks and conversations they will hear at cryo-EM meetings. Pre-requisites: The recommended prerequisites are college-freshman-level math, physics, and biochemistry. Pace: There are 14.5 hours of lecture videos total separated into 40 individual “modules” lasting on average 20 minutes each. Each module has at the end a list of “concept check” questions you can use to test your knowledge of what was presented. As the modules are grouped into seven major subjects, one reasonable plan would be to go through one major subject each day. That would mean watching a couple hours of lecture and spending another hour or so thinking through the concept check questions each day for a week. Another reasonable plan would be to go through one module each day for a little over a month, or even three modules a week (Monday, Wednesday, and Friday) for a 3-month term. It is likely that as you then move on to actually begin using a cryo-EM or otherwise engage in the field, you will want to repeat certain modules....

Melhores avaliações


18 de fev de 2018

Great course. I thought it was presented in a very informative way and i learned a lot about cryo-EM. In fact, it made me want to learn more about it. Thank you.


19 de jul de 2016

An excellent introduction to the topic, explaining from the basics to the detail of things. Useful and recommended to anyone in structural biology.

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26 — 50 de 51 Avaliações para o Introdução à Crio-Microscopia Eletrônica (Crio-ME)

por meera k m

22 de fev de 2016

very informative, simple and easily understandable. Love it

por sreenu b

11 de ago de 2016

Excellent class and Presentation with all clarity concepts

por Daniil B

14 de nov de 2015

Very nice description of electronic microscopy techniques

por Igor N

9 de jun de 2016

Really good introduction to cryo-EM and EM in general!

por Deleted A

9 de mar de 2021

Precise, in-depth and full of valuable knowledge.

por Vadim S

23 de jun de 2017

Great job! Clear explanations, drawings, schemes.

por Nivetha R

12 de out de 2019

Extremely Helpful and very comfortable to learn

por Xiaolong C

23 de dez de 2015

very great course, including so much details.

por Bing W

18 de mai de 2016

It's really useful. Thanks so much.

por 赵炜

25 de out de 2015

The best EM course available online

por ANIL K S

12 de set de 2018

very good for the beginner like me

por Baharak M

27 de dez de 2015

It" Awesome. Thank you.

por Chao-Han Y

28 de out de 2015


por Francois T

17 de ago de 2016

Thank you Dr. Jensen!

por 董炜哲

4 de set de 2015

absolutely fantastic!

por Janusz B

11 de abr de 2020

Fantastic lectures!

por jadson c d s

27 de dez de 2015


por Shokir P

13 de set de 2015

great course!

por stanwang

18 de out de 2017


por Scott C

29 de nov de 2015

Very good

por minagquan

9 de set de 2015


por Herman A

1 de fev de 2016

Great information, great teacher, greater visual presentation. Disclaimer: I only took the first week.

por Marcus G

1 de jan de 2016

Great introduction to Cryo-EM for biologists that covers all the basics in a clear and concise manner.

por Haris F

11 de jan de 2016

Pioneering online course in Electron Microscopy.

por Christine L

18 de out de 2016

Excellent content