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Voltar para Introdução à Crio-Microscopia Eletrônica (Crio-ME)

Comentários e feedback de alunos de Introdução à Crio-Microscopia Eletrônica (Crio-ME) da instituição Caltech – Instituto de Tecnologia da CalifórniaCaltech - Instituto de Tecnologia da Califórnia

4.9
221 classificações
42 avaliações

Sobre o curso

This class covers the fundamental principles underlying cryo-electron microscopy (cryo-EM) starting with the basic anatomy of electron microscopes, an introduction to Fourier transforms, and the principles of image formation. Building upon that foundation, the class then covers the sample preparation issues, data collection strategies, and basic image processing workflows for all 3 basic modalities of modern cryo-EM: tomography, single particle analysis, and 2-D crystallography. Philosophy: The course emphasizes concepts rather than mathematical details, taught through numerous drawings and example images. It is meant for anyone interested in the burgeoning fields of cryo-EM and 3-D EM, including cell biologists or molecular biologists without extensive training in mathematics or imaging physics and practicing electron microscopists who want to broaden their understanding of the field. The class is perfect as a primer for anyone who is about to be trained as a cryo-electron microscopist, or for anyone who needs an introduction to the field to be able to understand the literature or the talks and conversations they will hear at cryo-EM meetings. Pre-requisites: The recommended prerequisites are college-freshman-level math, physics, and biochemistry. Pace: There are 14.5 hours of lecture videos total separated into 40 individual “modules” lasting on average 20 minutes each. Each module has at the end a list of “concept check” questions you can use to test your knowledge of what was presented. As the modules are grouped into seven major subjects, one reasonable plan would be to go through one major subject each day. That would mean watching a couple hours of lecture and spending another hour or so thinking through the concept check questions each day for a week. Another reasonable plan would be to go through one module each day for a little over a month, or even three modules a week (Monday, Wednesday, and Friday) for a 3-month term. It is likely that as you then move on to actually begin using a cryo-EM or otherwise engage in the field, you will want to repeat certain modules....

Melhores avaliações

LR

Feb 19, 2018

Great course. I thought it was presented in a very informative way and i learned a lot about cryo-EM. In fact, it made me want to learn more about it. Thank you.

AL

Jul 20, 2016

An excellent introduction to the topic, explaining from the basics to the detail of things. Useful and recommended to anyone in structural biology.

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1 — 25 de {totalReviews} Avaliações para o Introdução à Crio-Microscopia Eletrônica (Crio-ME)

por Naďa L

Mar 22, 2019

Great course. Pity is that you don't give the certificate. Anyway, I'd like to thank to prof. Jensen for it.

por ANIL K S

Sep 12, 2018

very good for the beginner like me

por William N

Aug 31, 2018

Loved it. Although it's not heavy on math it helps to have a basic understanding of waves, how they propagate, how they are mathematically represented by sin/cos functions and how interference between two waves works.

por Gerardo M H

Mar 28, 2018

There is a few free courses, this one of the best that I've taken. It would be better if one finishes the course, one could receive any certification

por Lance R

Feb 19, 2018

Great course. I thought it was presented in a very informative way and i learned a lot about cryo-EM. In fact, it made me want to learn more about it. Thank you.

por Nikos N

Oct 30, 2017

CryoEM made crystal clear! ;P

por stanwang

Oct 19, 2017

excellent

por Vadim S

Jun 23, 2017

Great job! Clear explanations, drawings, schemes.

por Jonathan F

May 18, 2017

Great course, very informal - really improved my knowledge in the field of EM

por Robert F

Apr 04, 2017

Great course with clear explanations and very entertaining topics! Didn't expect to cover so many aspects of cryo-EM, would recommend it to everyone interested/working in structural biology or science in general.

por Serhiy S

Jan 26, 2017

I have worked a bit in cryo-em before and I find this course absolutely amazing! Very detailed and easy to understand.

por Jean N

Dec 14, 2016

Thank you for this valuable course, specially for the Fourrier image explanation.

por Christine L

Oct 19, 2016

Excellent content

por Francois T

Aug 17, 2016

Thank you Dr. Jensen!

por sreenu b

Aug 11, 2016

Excellent class and Presentation with all clarity concepts

por Aleix L

Jul 20, 2016

An excellent introduction to the topic, explaining from the basics to the detail of things. Useful and recommended to anyone in structural biology.

por Gary U

Jun 20, 2016

An excellent course to get to understand the field of cryogenic Electron Microscopy. The mathematics are presented at a high level, often geometrically, without getting into details. The physics of the electron microscope is presented again at a high level but with enough detail to understand the operation. The teaching style is well paced and inviting.

por Igor N

Jun 09, 2016

Really good introduction to cryo-EM and EM in general!

por Bing W

May 18, 2016

It's really useful. Thanks so much.

por Bryant A

May 16, 2016

Very well detailed on a easy to learn pase. Will help you learn all to EM that is necessary before using them.

por Pablo C

Mar 07, 2016

Great explanations and examples, even for complex things like the fourier space.

por meera k m

Feb 22, 2016

very informative, simple and easily understandable. Love it

por Herman A

Feb 01, 2016

Great information, great teacher, greater visual presentation. Disclaimer: I only took the first week.

por Haris F

Jan 11, 2016

Pioneering online course in Electron Microscopy.

por Marcus G

Jan 02, 2016

Great introduction to Cryo-EM for biologists that covers all the basics in a clear and concise manner.